MSH2 Recombinant Antibody

ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.", "Category"=>"Primary Antibody", "Clonality"=>"Recombinant", "Concentration"=>"Lot dependent", "Conjugation"=>"Unconjugated", "Cross Reactive Species"=>"Human", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bsm-60222R", "Gene ID"=>"4436", "Gene ID Link"=>"Entrez Gene: 4436", "Host"=>"Rabbit", "images"=>"[{\"id\":39500,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":5,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/50\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Tissue: Human tonsil\\r\\nSection type: Formalin fixed & Paraffin -embedded section\\r\\nRetrieval method: High temperature and high pressure\\r\\nRetrieval buffer: Tris\\/EDTA buffer, pH 9.0 Primary ab dilution: 1:50\\r\\nPrimary ab incubation condition: 1 hour at room temperature\\r\\nCounter stain: Hematoxylin\\r\\nComment: Color brown is the positive signal for bsm-60222R\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":2,\"disease_id\":2,\"sample_tissue_id\":22,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/39500_raw.jpg\",\"filepath_med\":\"20433\\/39500_med.jpg\",\"filepath_thumb\":\"20433\\/39500_thumb.jpg\",\"display_order\":2,\"lock_version\":1,\"created_at\":\"2022-03-07 00:56:02\",\"updated_at\":\"2022-03-07 00:56:02\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/39500\"},{\"id\":39501,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":5,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/50\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Tissue: Human esophagus\\r\\nSection type: Formalin fixed & Paraffin -embedded section\\r\\nRetrieval method: High temperature and high pressure\\r\\nRetrieval buffer: Tris\\/EDTA buffer, pH 9.0 Primary ab dilution: 1:50\\r\\nPrimary ab incubation condition: 1 hour at room temperature\\r\\nCounter stain: Hematoxylin\\r\\nComment: Color brown is the positive signal for bsm-60222R\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":3,\"disease_id\":2,\"sample_tissue_id\":28,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/39501_raw.jpg\",\"filepath_med\":\"20433\\/39501_med.jpg\",\"filepath_thumb\":\"20433\\/39501_thumb.jpg\",\"display_order\":3,\"lock_version\":1,\"created_at\":\"2022-03-07 00:56:28\",\"updated_at\":\"2022-03-07 00:56:29\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/39501\"},{\"id\":42735,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":1,\"application_note\":\"\",\"dilution\":\"1:1000\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Lane 1: Human HeLa cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human K562 cell lysates probed with MSH2 Monoclonal Antibody, Unconjugated (bsm-60222R) at 1:1000 dilution and 4\\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\",\"sample_type_id\":1,\"sample_details\":null,\"rating\":3,\"disease_id\":null,\"sample_tissue_id\":null,\"cell_line_id\":13,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/42735_raw.jpg\",\"filepath_med\":\"20433\\/42735_med.jpg\",\"filepath_thumb\":\"20433\\/42735_thumb.jpg\",\"display_order\":4,\"lock_version\":1,\"created_at\":\"2022-11-03 04:40:03\",\"updated_at\":\"2022-11-03 04:40:03\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/42735\"},{\"id\":49028,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":1,\"application_note\":\"\",\"dilution\":\"1:1000\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"25 ug total protein per lane of various lysates (see on figure) probed with MSH2 monoclonal antibody, unconjugated (bsm-60222R) at 1:1000 dilution and 4\\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. \",\"sample_type_id\":1,\"sample_details\":null,\"rating\":3,\"disease_id\":null,\"sample_tissue_id\":null,\"cell_line_id\":13,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49028_raw.jpg\",\"filepath_med\":\"20433\\/49028_med.jpg\",\"filepath_thumb\":\"20433\\/49028_thumb.jpg\",\"display_order\":5,\"lock_version\":1,\"created_at\":\"2024-09-23 01:55:36\",\"updated_at\":\"2024-09-23 01:55:36\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49028\"},{\"id\":49158,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":1,\"sample_tissue_id\":6,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49158_raw.jpg\",\"filepath_med\":\"20433\\/49158_med.jpg\",\"filepath_thumb\":\"20433\\/49158_thumb.jpg\",\"display_order\":6,\"lock_version\":1,\"created_at\":\"2024-09-26 04:06:35\",\"updated_at\":\"2024-09-26 04:06:35\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49158\"},{\"id\":49159,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":2,\"sample_tissue_id\":22,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":null,\"filepath_med\":null,\"filepath_thumb\":null,\"display_order\":7,\"lock_version\":0,\"created_at\":\"2024-09-26 04:07:11\",\"updated_at\":\"2024-09-26 04:07:11\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49159\"},{\"id\":49160,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":2,\"sample_tissue_id\":20,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49160_raw.jpg\",\"filepath_med\":\"20433\\/49160_med.jpg\",\"filepath_thumb\":\"20433\\/49160_thumb.jpg\",\"display_order\":8,\"lock_version\":1,\"created_at\":\"2024-09-26 04:07:53\",\"updated_at\":\"2024-09-26 04:07:53\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49160\"},{\"id\":49161,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":3,\"disease_id\":2,\"sample_tissue_id\":22,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49161_raw.jpg\",\"filepath_med\":\"20433\\/49161_med.jpg\",\"filepath_thumb\":\"20433\\/49161_thumb.jpg\",\"display_order\":9,\"lock_version\":1,\"created_at\":\"2024-09-26 04:08:40\",\"updated_at\":\"2024-09-26 04:08:40\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49161\"},{\"id\":49162,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":2,\"sample_tissue_id\":6,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49162_raw.jpg\",\"filepath_med\":\"20433\\/49162_med.jpg\",\"filepath_thumb\":\"20433\\/49162_thumb.jpg\",\"display_order\":10,\"lock_version\":1,\"created_at\":\"2024-09-26 04:09:06\",\"updated_at\":\"2024-09-26 04:09:06\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49162\"},{\"id\":49163,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":2,\"sample_tissue_id\":28,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49163_raw.jpg\",\"filepath_med\":\"20433\\/49163_med.jpg\",\"filepath_thumb\":\"20433\\/49163_thumb.jpg\",\"display_order\":11,\"lock_version\":1,\"created_at\":\"2024-09-26 04:09:34\",\"updated_at\":\"2024-09-26 04:09:34\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49163\"},{\"id\":49164,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Endometrial ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":2,\"sample_tissue_id\":22,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49164_raw.jpg\",\"filepath_med\":\"20433\\/49164_med.jpg\",\"filepath_thumb\":\"20433\\/49164_thumb.jpg\",\"display_order\":12,\"lock_version\":1,\"created_at\":\"2024-09-26 04:10:03\",\"updated_at\":\"2024-09-26 04:10:04\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49164\"},{\"id\":49165,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":3,\"disease_id\":1,\"sample_tissue_id\":22,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49165_raw.jpg\",\"filepath_med\":\"20433\\/49165_med.jpg\",\"filepath_thumb\":\"20433\\/49165_thumb.jpg\",\"display_order\":13,\"lock_version\":1,\"created_at\":\"2024-09-26 04:10:29\",\"updated_at\":\"2024-09-26 04:10:29\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49165\"}]", "Isotype"=>"IgG", "Lead Time"=>"3 to 5 business days.", "Modification"=>"Unmodified", "Notes"=>"CY7070", "Product URL"=>"https://www.biossusa.com/products/bsm-60222R", "Purification"=>"Purified by Protein A.", "Size"=>"100ul", "Source"=>"KLH conjugated synthetic peptide derived from human MSH2", "Storage"=>"0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.", "Storage Buffer"=>"0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol", "Storage Condition"=>"Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.", "Subcellular Locations"=>"Nucleus", "Swiss Prot"=>"P43246", "Swiss Prot Link"=>"Swiss Prot: P43246", "Synonyms"=>"MSH-2; BAT26; DNA mismatch repair protein Msh2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1; BAT26; COCA 1; COCA1; DNA mismatch repair protein Msh2; FCC 1; FCC1; hMSH2; HNPCC 1; HNPCC; HNPCC1; LCFS2; MSH 2; Msh2; MSH2_HUMAN; MutS homolog 2; MutS homolog 2 colon cancer nonpolyposis type 1; MutS protein homolog 2.", "Target Protein"=>"MSH2"} -->