MSH2 Recombinant Antibody

ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.", "Category"=>"Primary Antibody", "Clonality"=>"Recombinant", "Concentration"=>"Lot dependent", "Conjugation"=>"Unconjugated", "Cross Reactive Species"=>"Human", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bsm-60222R", "Gene ID"=>"4436", "Gene ID Link"=>"Entrez Gene: 4436", "Host"=>"Rabbit", "images"=>"[{\"id\":49158,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":1,\"sample_tissue_id\":6,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49158_raw.jpg\",\"filepath_med\":\"20433\\/49158_med.jpg\",\"filepath_thumb\":\"20433\\/49158_thumb.jpg\",\"display_order\":6,\"lock_version\":3,\"created_at\":\"2024-09-26 04:06:35\",\"updated_at\":\"2025-08-25 03:52:48\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49158\"},{\"id\":49159,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":2,\"sample_tissue_id\":22,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49159_raw.jpg\",\"filepath_med\":\"20433\\/49159_med.jpg\",\"filepath_thumb\":\"20433\\/49159_thumb.jpg\",\"display_order\":7,\"lock_version\":2,\"created_at\":\"2024-09-26 04:07:11\",\"updated_at\":\"2025-08-25 03:54:19\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49159\"},{\"id\":49160,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":2,\"sample_tissue_id\":20,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49160_raw.jpg\",\"filepath_med\":\"20433\\/49160_med.jpg\",\"filepath_thumb\":\"20433\\/49160_thumb.jpg\",\"display_order\":8,\"lock_version\":3,\"created_at\":\"2024-09-26 04:07:53\",\"updated_at\":\"2025-08-25 03:57:29\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49160\"},{\"id\":49161,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":2,\"sample_tissue_id\":22,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49161_raw.jpg\",\"filepath_med\":\"20433\\/49161_med.jpg\",\"filepath_thumb\":\"20433\\/49161_thumb.jpg\",\"display_order\":9,\"lock_version\":3,\"created_at\":\"2024-09-26 04:08:40\",\"updated_at\":\"2025-08-25 03:58:36\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49161\"},{\"id\":49162,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":2,\"sample_tissue_id\":6,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49162_raw.jpg\",\"filepath_med\":\"20433\\/49162_med.jpg\",\"filepath_thumb\":\"20433\\/49162_thumb.jpg\",\"display_order\":10,\"lock_version\":3,\"created_at\":\"2024-09-26 04:09:06\",\"updated_at\":\"2025-08-25 04:01:02\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49162\"},{\"id\":49163,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":3,\"disease_id\":2,\"sample_tissue_id\":28,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49163_raw.jpg\",\"filepath_med\":\"20433\\/49163_med.jpg\",\"filepath_thumb\":\"20433\\/49163_thumb.jpg\",\"display_order\":11,\"lock_version\":3,\"created_at\":\"2024-09-26 04:09:34\",\"updated_at\":\"2025-08-25 04:02:56\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49163\"},{\"id\":49164,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Endometrial ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":2,\"sample_tissue_id\":22,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49164_raw.jpg\",\"filepath_med\":\"20433\\/49164_med.jpg\",\"filepath_thumb\":\"20433\\/49164_thumb.jpg\",\"display_order\":12,\"lock_version\":3,\"created_at\":\"2024-09-26 04:10:03\",\"updated_at\":\"2025-08-25 04:04:20\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49164\"},{\"id\":49165,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":4,\"application_note\":\"\",\"dilution\":\"1\\/200\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MSH2 Monoclonal Antibody, Unconjugated(bsm-60222R) at 1:200 overnight at 4\\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.\",\"sample_type_id\":2,\"sample_details\":null,\"rating\":4,\"disease_id\":1,\"sample_tissue_id\":3,\"cell_line_id\":null,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/49165_raw.jpg\",\"filepath_med\":\"20433\\/49165_med.jpg\",\"filepath_thumb\":\"20433\\/49165_thumb.jpg\",\"display_order\":13,\"lock_version\":3,\"created_at\":\"2024-09-26 04:10:29\",\"updated_at\":\"2025-08-25 04:05:24\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/49165\"},{\"id\":60896,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":1,\"application_note\":\"\",\"dilution\":\"1:1000\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"25 ug total protein per lane of various lysates (see on figure) probed with MSH2 monoclonal antibody, unconjugated (bsm-60222R) at 1:1000 dilution and 4\\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. \",\"sample_type_id\":1,\"sample_details\":null,\"rating\":3,\"disease_id\":null,\"sample_tissue_id\":null,\"cell_line_id\":13,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/60896_raw.jpg\",\"filepath_med\":\"20433\\/60896_med.jpg\",\"filepath_thumb\":\"20433\\/60896_thumb.jpg\",\"display_order\":14,\"lock_version\":3,\"created_at\":\"2025-08-21 04:29:05\",\"updated_at\":\"2025-08-25 21:47:05\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/60896\"},{\"id\":61013,\"status\":\"active\",\"visibility\":\"public\",\"origin_id\":1,\"origin_notes\":\"\",\"product_id\":20433,\"productconjugation_id\":null,\"application_id\":3,\"application_note\":\"\",\"dilution\":\"1:100\",\"cross_reactive_species_id\":1,\"cross_reactive_species_note\":\"\",\"description\":\"The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MSH2 antibody (bsm-60222R): 1 \\u03bcg\\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\",\"sample_type_id\":1,\"sample_details\":null,\"rating\":5,\"disease_id\":null,\"sample_tissue_id\":null,\"cell_line_id\":13,\"protocols_used\":\"\",\"additional_notes\":\"\",\"filepath_raw\":\"20433\\/61013_raw.jpg\",\"filepath_med\":\"20433\\/61013_med.jpg\",\"filepath_thumb\":\"20433\\/61013_thumb.jpg\",\"display_order\":15,\"lock_version\":1,\"created_at\":\"2025-08-25 05:04:59\",\"updated_at\":\"2025-08-25 05:04:59\",\"image_url\":\"http:\\/\\/biossantibodies.com\\/image-raw\\/61013\"}]", "Isotype"=>"IgG", "Lead Time"=>"3 to 5 business days.", "Modification"=>"Unmodified", "Product URL"=>"https://www.biossusa.com/products/bsm-60222R", "Promo"=>"Promo", "Purification"=>"Purified by Protein A.", "Size"=>"100ul", "Source"=>"KLH conjugated synthetic peptide derived from human MSH2", "Storage"=>"0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.", "Storage Buffer"=>"0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol", "Storage Condition"=>"Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.", "Subcellular Locations"=>"Nucleus", "Swiss Prot"=>"P43246", "Swiss Prot Link"=>"Swiss Prot: P43246", "Synonyms"=>"MSH-2; BAT26; DNA mismatch repair protein Msh2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1; BAT26; COCA 1; COCA1; DNA mismatch repair protein Msh2; FCC 1; FCC1; hMSH2; HNPCC 1; HNPCC; HNPCC1; LCFS2; MSH 2; Msh2; MSH2_HUMAN; MutS homolog 2; MutS homolog 2 colon cancer nonpolyposis type 1; MutS protein homolog 2.", "Target Protein"=>"MSH2"} -->