Kallikrein 5 Monoclonal Antibody

$MCF-7 cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Kallikrein 5) monoclonal Antibody, Unconjugated (bsm-54746R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

MCF-7 cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Kallikrein 5) monoclonal Antibody, Unconjugated (bsm-54746R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

A431 cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Kallikrein 5 Monoclonal Antibody(bsm-54746R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-54746R
Product Name	Kallikrein 5 Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide corresponding to Human Kallikrein 5.
Clonality	Monoclonal
Clone #	9H4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Swiss Prot	Q9Y337
Subcellular location	Secreted
Synonyms	HK5 antibody, Kallikrein 5 antibody, Kallikrein L2 antibody, Kallikrein like 2 antibody, Kallikrein like protein 2 antibody, Kallikrein related peptidase 5 antibody, Kallikrein-5 antibody, Kallikrein-like protein 2 antibody, Kallikrein5 antibody, KLK 5 antibody, KLK L2 antibody, KLK-L2 antibody, KLK5 antibody, KLK5_HUMAN antibody, KLKL 2 antibody, KLKL2 antibody, SCTE antibody, Stratum corneum tryptic enzyme antibody, UNQ570/PRO1132 antibody
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200