GSTK1 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GSTK1 Monoclonal Antibody, Unconjugated (bsm-54652R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Flow cytometric analysis of GSTK1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54652R, 1\/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1\/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).$

Flow cytometric analysis of GSTK1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54652R, 1\/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1\/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human

Overview
Catalog #	bsm-54652R
Product Name	GSTK1 Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within N terminal human GSTK1.
Clonality	Monoclonal
Clone #	1G8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	373156
Swiss Prot	Q9Y2Q3
Subcellular location	Peroxisome
Synonyms	EC 2.5.1.18 antibody, Glutathione S Transferase kappa 1 antibody, Glutathione S transferase subunit 13 antibody, Glutathione S-transferase k1 antibody, Glutathione S-transferase kappa 1 antibody, Glutathione S-transferase subunit 13 antibody, Glutathione S-transferase subunit 13 homolog antibody, GST 13 13 antibody, GST 13-13 antibody, GST antibody, GST class kappa antibody, GST class-kappa antibody, GST13 antibody, GST13-13 antibody, GSTK1 1 antibody, Gstk1 antibody, GSTK1-1 antibody, GSTK1_HUMAN antibody, hGSTK1 antibody
Background	Members of the glutathione S-transferase (GST) family of proteins function in the detoxification of xenobiotics to protect cells against toxicant-induced damage. There are eight families of GST proteins, namely alpha, zeta, theta, kappa, mu, pi, sigma and omega, each of which are composed of proteins that have a variety of functions throughout the cell. GSTK1 (glutathione S-transferase kappa 1), also known as glutathione S-transferase subunit 13 (GST 13-13) or GSTK1-1, is a 226 amino acid ubiquitously expressed protein belonging to the kappa class of the GST superfamily that functions in cellular detoxification. Localizing to peroxisome, GSTK1 exists as a homodimer that catalyzes the conjugation of glutathione to a number of hydrophobic substrates leading to their removal from the cell.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC