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Phospho-PKA R2 (S99) Monoclonal Antibody

Applications

  • WB
  • IHC-P
  • IF(ICC)
  • IP
  • IHC

Reactivity

  • Human
  • Mouse
  • Rat
  • Pig
Overview
Catalog # bsm-54545R
Product Name Phospho-PKA R2 (S99) Monoclonal Antibody
Applications WB, IHC-P, IF(ICC), IP, IHC
Reactivity Human, Mouse, Rat, Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein
Modification Site Ser99
Clonality Monoclonal
Clone # 4H11
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 5576
Swiss Prot P13861
Subcellular location Cytoplasm, Cell membrane
Synonyms cAMP dependent protein kinase regulatory subunit alpha 2 antibody, cAMP dependent protein kinase regulatory subunit RII alpha antibody, cAMP dependent protein kinase type II alpha regulatory chain antibody, cAMP dependent protein kinase type II alpha regulatory subunit antibody, cAMP-dependent protein kinase type II-alpha regulatory subunit antibody, KAP2 antibody, KAP2_HUMAN antibody, MGC3606 antibody, PKR 2 antibody, PKR2 antibody, PRKA R2 antibody, PRKAR 2 antibody, PRKAR2 antibody, PRKAR2A antibody, Protein kinase A RII alpha subunit antibody, Protein kinase cAMP dependent regulatory type II alpha antibody
Background The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme, resulting in release of active catalytic subunits. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus, where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the Pol II transcription initiation complex. Mouse Serine 96 (designated Ser 99 in human) is a phosphorylation site on the PKA II?regulatory subunit
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IP 1-2ug
IHC