HIF-1 beta Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HIF-1 beta Monoclonal Antibody, Unconjugated (bsm-54534R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HIF-1 beta Monoclonal Antibody, Unconjugated (bsm-54534R) at 1:50 for 30 minutes at room temperature, DAB staining.

Applications

WB
IHC-P
IHC

Reactivity

Human

Overview
Catalog #	bsm-54534R
Product Name	HIF-1 beta Monoclonal Antibody
Applications	WB, IHC-P, IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein
Clonality	Monoclonal
Clone #	11H1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	405
Swiss Prot	P27540
Subcellular location	Nucleus
Synonyms	arnT antibody, ARNT protein antibody, ARNT_HUMAN antibody, Aryl hydrocarbon receptor nuclear translocator antibody, bHLHe2 antibody, Class E basic helix-loop-helix protein 2 antibody, Dioxin receptor antibody, Dioxin receptor nuclear translocator antibody, Drnt antibody, HIF 1 beta antibody, HIF 1beta antibody, HIF-1-beta antibody, HIF1-beta antibody, HIF1B antibody, HIF1beta antibody, Hypoxia Inducible Factor 1 antibody, Hypoxia inducible factor 1 beta antibody, Hypoxia-inducible factor 1-beta antibody, nuclear translocator antibody, Tango antibody
Background	This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC