CD79a (1B12) Monoclonal Antibody

Flow cytometric analysis of Daudi cells with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.$

Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

$Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.$

Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

$Lane 1: Daudi Cells; Lane 2: Raji Cells; Probed with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Daudi Cells; Lane 2: Raji Cells; Probed with CD79a (1B12) Monoclonal Antibody (bsm-54458R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-54458R
Product Name	CD79a (1B12) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human CD79a aa 33-143.
Clonality	Monoclonal
Clone #	1B12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	973
Swiss Prot	P11912
Subcellular location	Extracellular, Cell membrane
Synonyms	B-cell antigen receptor complex-associated protein alpha chain; CD79a; Ig-alpha; IGA; MB1; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein
Background	Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400