IMPDH2 (2H4) Monoclonal Antibody

$Lane 1: Daudi Cells; Probed with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Daudi Cells; Probed with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Thyroid tissue incubated with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Thyroid tissue incubated with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Daudi cells with IMPDH2 (2H4) Monoclonal Antibody (bsm-54443R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54443R
Product Name	IMPDH2 (2H4) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC), IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within C-terminal human IMPDH2.
Clonality	Monoclonal
Clone #	2H4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3615
Swiss Prot	P12268
Subcellular location	Cytoplasm, Nucleus
Synonyms	Inosine-5'-monophosphate dehydrogenase 2; IMPDH-II, IMP dehydrogenase 2; IMPD 2; IMPDH 2; IMPDH2; IMPD2
Background	Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200
IP	1-2ug