FKBP4 (12A5) Monoclonal Antibody

$Lane 1: 293 Cells; Lane 2: SK-Br-3 Cells; Probed with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: 293 Cells; Lane 2: SK-Br-3 Cells; Probed with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Esophagus tissue incubated with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Esophagus tissue incubated with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Brain tissue incubated with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of 293T cells with FKBP4 (12A5) Monoclonal Antibody (bsm-54422R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54422R
Product Name	FKBP4 (12A5) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human FKBP52 aa 1-200.
Clonality	Monoclonal
Clone #	12A5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2288
Swiss Prot	Q02790
Subcellular location	Cytoplasm, Nucleus, Mitochondrion, Cytoskeleton
Synonyms	Peptidyl-prolyl cis-trans isomerase FKBP4; 51 kDa FK506-binding protein; 52 kDa FK506-binding protein; 59 kDa immunophilin; FK506-binding protein 4; FKBP59; HSP-binding immunophilin; Immunophilin FKBP52; Rotamase; Peptidyl-prolyl cis-trans isomerase FKBP4 N-terminally processed; PPIase FKBP4; FKBP51; 52 kDa FKBP; FKBP-52; p59; FKBP-4; HBI
Background	Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200