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CBFB (5D4) Monoclonal Antibody

Applications

  • WB
  • FCM
  • IHC-P

Reactivity

  • Human
  • Rat
Overview
Catalog # bsm-54418R
Product Name CBFB (5D4) Monoclonal Antibody
Applications WB, FCM, IHC-P
Reactivity Human, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein within human CBFb aa 1-200.
Clonality Monoclonal
Clone # 5D4
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 865
Swiss Prot Q13951
Subcellular location Nucleus
Synonyms Core-binding factor subunit beta; Polyomavirus enhancer-binding protein 2 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; CBF-beta; PEA2-beta; PEBP2-beta; CBFB
Background Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400