EGR2 (5C5) Monoclonal Antibody

$Lane 1: MCF-7 Cells; Lane 2: SH-SY5Y Cells; Probed with EGR2 (5C5) Monoclonal Antibody (bsm-54391R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MCF-7 Cells; Lane 2: SH-SY5Y Cells; Probed with EGR2 (5C5) Monoclonal Antibody (bsm-54391R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Flow cytometric analysis of SH-SY5Y cells with EGR2 (5C5) Monoclonal Antibody (bsm-54391R) at a 1:50 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54391R
Product Name	EGR2 (5C5) Monoclonal Antibody
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human EGR2 aa 1-200.
Clonality	Monoclonal
Clone #	5C5
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Swiss Prot	P11161
Subcellular location	Nucleus
Synonyms	E3 SUMO-protein ligase EGR2; AT591; E3 SUMO-protein transferase ERG2; Early growth response protein 2; Zinc finger protein Krox-20; EGR-2; EGR2; KROX20
Background	The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Binds to two specific DNA sites located in the promoter region of HOXA4. E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 own transcriptional activity. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene.
Application Dilution
WB	1:300-5000
FCM	1:20-100