VAMP3 (8A3) Monoclonal Antibody

$Lane 1: A549 Cells; Lane 2: 293T Cells; Probed with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: A549 Cells; Lane 2: 293T Cells; Probed with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:1000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of SiHa cells with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Hippocampus tissue incubated with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Hippocampus tissue incubated with VAMP3 (8A3) Monoclonal Antibody (bsm-54366R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54366R
Product Name	VAMP3 (8A3) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide conjugated to KLH within N-terminal human VAMP3/Cellubrevin.
Clonality	Monoclonal
Clone #	8A3
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	9341
Swiss Prot	Q15836
Subcellular location	Cytoplasm, Membrane bound
Synonyms	Vesicle-associated membrane protein 3; Cellubrevin; Synaptobrevin-3; VAMP-3; CEB; SYB3
Background	Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP3 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. VAMP3 is involved in vesicular transport from the late endosomes to the trans-Golgi network.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200