UQCRFS1 (10C1) Monoclonal Antibody

Flow cytometric analysis of A549 cells with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Appendix tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Lane 1: A549 Cells; Lane 2: 293 Cells; Lane 3: Mouse Kidney; Probed with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: A549 Cells; Lane 2: 293 Cells; Lane 3: Mouse Kidney; Probed with UQCRFS1 (10C1) Monoclonal Antibody (bsm-54363R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54363R
Product Name	UQCRFS1 (10C1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human UQCRFS1 aa 100 to the C-terminus.
Clonality	Monoclonal
Clone #	10C1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	7386
Swiss Prot	P47985
Subcellular location	Mitochondrion
Synonyms	Cytochrome b-c1 complex subunit Rieske mitochondrial; Complex III subunit 5; Cytochrome b-c1 complex subunit 5; Rieske iron-sulfur protein; Rieske protein UQCRFS1; Ubiquinol-cytochrome c reductase iron-sulfur subunit; Cytochrome b-c1 complex subunit 9; Complex III subunit IX; Cytochrome b-c1 complex subunit 11; Ubiquinol-cytochrome c reductase 8 kDa protein; RISP
Background	Cytochrome b-c1 complex subunit Rieske, mitochondrial: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. Incorporation of UQCRFS1 is the penultimate step in complex III assembly.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200