Syntrophin alpha 1 (2A1) Monoclonal Antibody

$Lane 1: SiHa Cells; Probed with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SiHa Cells; Probed with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Skeletal Muscle tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Skeletal Muscle tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of LOVO cells with Syntrophin alpha 1 (2A1) Monoclonal Antibody (bsm-54351R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54351R
Product Name	Syntrophin alpha 1 (2A1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Syntrophin alpha 1 aa 1-200.
Clonality	Monoclonal
Clone #	2A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	6640
Swiss Prot	Q13424
Subcellular location	Cell membrane, Cytoskeleton
Synonyms	Alpha-1-syntrophin; 59 kDa dystrophin-associated protein A1 acidic component 1; Pro-TGF-alpha cytoplasmic domain-interacting protein 1; Syntrophin-1; TACIP1; SNTA1; SNT1
Background	Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the extracellular matrix via the dystrophin glycoprotein complex. Plays an important role in synapse formation and in the organization of UTRN and acetylcholine receptors at the neuromuscular synapse. Binds to phosphatidylinositol 4,5-bisphosphate.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200