Creatine Kinase B (1D8) Monoclonal Antibody

$Lane 1: Mouse Brain; Lane 2: Mouse Brain; Lane 3: Rat Brain; Lane 4: 293 Cells; Probed with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Brain; Lane 2: Mouse Brain; Lane 3: Rat Brain; Lane 4: 293 Cells; Probed with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Prostate tissue incubated with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of 293T cells with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54297R
Product Name	Creatine Kinase B (1D8) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Creatine kinase B type aa 1-150
Clonality	Monoclonal
Clone #	1D8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	1152
Swiss Prot	P12277
Synonyms	Creatine kinase B-type; Brain creatine kinase; Creatine kinase B chain; Creatine phosphokinase B-type; BCK; CPK-B; CKB; CKBB
Background	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200