PDK2 (2F4) Monoclonal Antibody

$Lane 1: Rat Heart; Probed with PDK2 (2F4) Monoclonal Antibody (bsm-54291R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Rat Heart; Probed with PDK2 (2F4) Monoclonal Antibody (bsm-54291R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with PDK2 (2F4) Monoclonal Antibody (bsm-54291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with PDK2 (2F4) Monoclonal Antibody (bsm-54291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with PDK2 (2F4) Monoclonal Antibody (bsm-54291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with PDK2 (2F4) Monoclonal Antibody (bsm-54291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with PDK2 (2F4) Monoclonal Antibody (bsm-54291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with PDK2 (2F4) Monoclonal Antibody (bsm-54291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with PDK2 (2F4) Monoclonal Antibody (bsm-54291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with PDK2 (2F4) Monoclonal Antibody (bsm-54291R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of SH-SY5Y cells with PDK2 (2F4) Monoclonal Antibody (bsm-54291R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54291R
Product Name	PDK2 (2F4) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human PDK2 aa 200-400
Clonality	Monoclonal
Clone #	2F4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	5164
Swiss Prot	Q15119
Subcellular location	Mitochondrion
Synonyms	Pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 2 mitochondrial; Pyruvate dehydrogenase kinase 2; PDH kinase 2; PDKII; PDK2; PDHK2
Background	Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200