EPAS1 Recombinant Antibody

$Lane 1: SiHa Cells; Probed with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: SiHa Cells; Probed with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Lung tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Breast Cancer tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of HUVEC cells with EPAS1 (2G3) Monoclonal Antibody (bsm-54278R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Rat Testis tissue lysates ; Lane 5: Human HepG2 cell lysates; Lane 6:Human MCF-7 cell lysates; Lane 7: Human U87MG cell lysates; Lane 8: Human SH-SY5Y cell lysates probed with EPAS1 (2G3) Monoclonal Antibody, Unconjugated (bsm-54278R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54278r-100ul
Product Name	EPAS1 Recombinant Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant full length Human protein
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2034
Swiss Prot	Q99814
Subcellular location	Nucleus
Synonyms	HIF-2 alpha; Endothelial PAS domain-containing protein 1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; HIF-1-alpha-like factor; Hypoxia-inducible factor 2-alpha; PAS domain-containing protein 2; EPAS-1; bHLHe73; HLF; HIF2-alpha; EPAS1; BHLHE73; HIF2A; MOP2; PASD2; ECYT4
Background	Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400