DNA Ligase I (3G2) Monoclonal Antibody

$Lane 1: Daudi Cells; Lane 2: A431 Cells; Lane 3: MCF-7 Cells; Probed with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Daudi Cells; Lane 2: A431 Cells; Lane 3: MCF-7 Cells; Probed with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human
Rat

Overview
Catalog #	bsm-54261R
Product Name	DNA Ligase I (3G2) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Clonality	Monoclonal
Clone #	3G2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	3978
Swiss Prot	P18858
Subcellular location	Nucleus
Synonyms	DNA ligase 1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1; LIG1.
Background	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400