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VPS35 (7C4) Monoclonal Antibody

Applications

  • WB
  • FCM
  • IHC-P

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-54248R
Product Name VPS35 (7C4) Monoclonal Antibody
Applications WB, FCM, IHC-P
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant protein within human VPS35 1-150
Clonality Monoclonal
Clone # 7C4
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID 55737
Swiss Prot Q96QK1
Subcellular location Cytoplasm, Membrane bound, Endosome
Synonyms Vacuolar protein sorting-associated protein 35; Maternal-embryonic 3; Vesicle protein sorting 35; hVPS35; VPS35; MEM3; TCCCTA00141; PARK17; VPS35 retromer complex component.
Background Vacuolar sorting proteins (VPSs) are required for proper trafficking of endocytic and biosynthetic proteins to the vacuole and play an important role in the budding process of cells. VPS35 (vacuolar protein sorting 35), also known as MEM3, is the 796 amino acid human homolog of the S. cerevisiae Vps35 protein. Localized to the cytoplasm and to the peripheral membrane, VPS35 is an essential component of the retromer complex which is involved in retrieval of lysosomal enzyme receptors from endosomes to the trans-Golgi network. VPS35 is expressed ubiquitously with highest expression in heart, placenta, brain, testis, kidney, colon, ovary and spleen. In addition to its crucial role in the retromer complex, VPS35 is part of a subcomplex that is required to regulate transcytosis of the polymeric immunoglobulin receptor from the basolateral to the apical surface of epithelial cells and hepatocytes.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400