PTGES3 (1A26) Monoclonal Antibody

$Lane 1: Mouse Brain; Lane 2: SK-Br-3 Cells; Lane 3: Rat Lung; Probed with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Brain; Lane 2: SK-Br-3 Cells; Lane 3: Rat Lung; Probed with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Fallopian Tube tissue incubated with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Fallopian Tube tissue incubated with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)
IP
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54193R
Product Name	PTGES3 (1A26) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IP, IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within human p23 aa 50-100
Clonality	Monoclonal
Clone #	1A26
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	10728
Swiss Prot	Q15185
Synonyms	Prostaglandin E synthase 3; Cytosolic prostaglandin E2 synthase; Hsp90 co-chaperone; Progesterone receptor complex p23; Telomerase-binding protein p23; cPGES; P23; TEBP.
Background	Cytosolic prostaglandin synthase that catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. Facilitates HIF alpha proteins hydroxylation via interaction with EGLN1/PHD2, leading to recruit EGLN1/PHD2 to the HSP90 pathway.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IP	1-2ug
IHC