Transglutaminase 2 (2C2) Monoclonal Antibody

$Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Transglutaminase 2 (2C2) Monoclonal Antibody (bsm-54185R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with Transglutaminase 2 (2C2) Monoclonal Antibody (bsm-54185R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: HUVEC Cells; Probed with Transglutaminase 2 (2C2) Monoclonal Antibody (bsm-54185R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HUVEC Cells; Probed with Transglutaminase 2 (2C2) Monoclonal Antibody (bsm-54185R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: Mouse Placenta; Lane 2: Mouse Lung; Probed with Transglutaminase 2 (2C2) Monoclonal Antibody (bsm-54185R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Placenta; Lane 2: Mouse Lung; Probed with Transglutaminase 2 (2C2) Monoclonal Antibody (bsm-54185R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54185R
Product Name	Transglutaminase 2 (2C2) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within human Transglutaminase 2 aa 550-600
Clonality	Monoclonal
Clone #	2C2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	7052
Swiss Prot	P21980
Synonyms	Protein-glutamine gamma-glutamyltransferase 2; Tissue transglutaminase; Transglutaminase C; Transglutaminase H; Transglutaminase-2; TG(C); TGC; TGase C; TGase H; TGase-2; TGM2.
Background	Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400