Vinculin (5G7) Monoclonal Antibody

$Lane 1: HepG2 Cells; Lane 2: Human Fetal Skeletal Muscle; Lane 3: Rat Spleen; lane 4: Mouse Kidney; Probed with Vinculin (5G7) Monoclonal Antibody (bsm-54148R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HepG2 Cells; Lane 2: Human Fetal Skeletal Muscle; Lane 3: Rat Spleen; lane 4: Mouse Kidney; Probed with Vinculin (5G7) Monoclonal Antibody (bsm-54148R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Vinculin (5G7) Monoclonal Antibody (bsm-54148R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Vinculin (5G7) Monoclonal Antibody (bsm-54148R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Vinculin (5G7) Monoclonal Antibody (bsm-54148R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Vinculin (5G7) Monoclonal Antibody (bsm-54148R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Vinculin (5G7) Monoclonal Antibody (bsm-54148R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Vinculin (5G7) Monoclonal Antibody (bsm-54148R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Lane 1: Mouse Skeletal muscle lysates; Lane 2: Rat Skeletal muscle lysates; Lane 3: Mouse Urinary bladder lysates; Lane 4: Rat Urinary bladder lysates; Lane 5: Human U251 cell lysates; Lane 6: Human HUVEC cell lysates; Lane 7: Human A431 cell lysates; Lane 8: Human HepG2 cell lysates; Lane 9: Mouse Spleen lysates; Lane 10: Rat Spleen lysates; Lane 11: Mouse Kidney lysates; Lane 12: Human A549 cell lysates probed with Vinculin (5G7) Monoclonal Antibody, Unconjugated (bsm-54148R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Testis tissue lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human K562 cell lysates; Lane 5: Human HUVEC cell lysates probed with Vinculin (Loading Control) Monoclonal Antibody, Unconjugated (bsm-54148R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human K562 cell lysates; Lane 5: Human HUVEC cell lysates probed with Vinculin (Loading Control) Monoclonal Antibody, Unconjugated (bsm-54148R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54148R
Product Name	Vinculin (5G7) Monoclonal Antibody
Applications	WB, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within human Vinculin aa 1000-1100
Clonality	Monoclonal
Clone #	5G7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7414
Swiss Prot	P18206
Synonyms	Vinculin; Metavinculin; MV; VCL; MVCL; CMD1W; CMH15; HEL114.
Background	Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in Vinculin are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200