Thymidylate Synthase (6C2) Monoclonal Antibody

$Lane 1: Hela Cells; Lane 2: Jurkat Cells; Lane 3: Raji Cells; Lane 4: Mouse Thymus; Probed with Thymidylate Synthase (6C2) Monoclonal Antibody (bsm-54082R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cells; Lane 2: Jurkat Cells; Lane 3: Raji Cells; Lane 4: Mouse Thymus; Probed with Thymidylate Synthase (6C2) Monoclonal Antibody (bsm-54082R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with Thymidylate Synthase (6C2) Monoclonal Antibody (bsm-54082R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with Thymidylate Synthase (6C2) Monoclonal Antibody (bsm-54082R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Thymidylate Synthase (6C2) Monoclonal Antibody (bsm-54082R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with Thymidylate Synthase (6C2) Monoclonal Antibody (bsm-54082R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Bone tissue incubated with Thymidylate Synthase (6C2) Monoclonal Antibody (bsm-54082R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Bone tissue incubated with Thymidylate Synthase (6C2) Monoclonal Antibody (bsm-54082R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Hela cells with Thymidylate Synthase (6C2) Monoclonal Antibody (bsm-54082R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54082R
Product Name	Thymidylate Synthase (6C2) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Thymidylate Synthase aa 300 to the C-terminus
Clonality	Monoclonal
Clone #	6C2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	7298
Swiss Prot	P04818
Synonyms	Thymidylate synthase; TS; TSase; TYMS; OK/SW-cl.29; HST422
Background	Thymidylate synthase (TS), also designated TYMS, TMS, TSase and HsT422, uses 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor in the synthesis of 2'-deoxythymidine-5'-monophosphate (dTMP), an essential precursor for DNA biosynthesis. TS is an RNA-binding protein that can interact with its own mRNA. The TS/mRNA ribonucleoprotein complex can also associate with a number of other cellular mRNAs, including those corresponding to the p53 tumor suppressor gene and the Myc family of transcription factors. Inhibition of DNA replication and cell death resulting from thymidine depletion occurs when TS enzyme activity is inhibited with substrate or cofactor analogs, making the TS enzyme an important target for chemotherapy. Cancer cells are sensitive to thymidine depletion, as they multiply rapidly
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200