SPHK1 (3G1) Monoclonal Antibody

$Lane 1: C2C12 Cells; Probed SPHK1 (3G1) Monoclonal Antibody (bsm-54045R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: C2C12 Cells; Probed SPHK1 (3G1) Monoclonal Antibody (bsm-54045R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated SPHK1 (3G1) Monoclonal Antibody (bsm-54045R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated SPHK1 (3G1) Monoclonal Antibody (bsm-54045R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated SPHK1 (3G1) Monoclonal Antibody (bsm-54045R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated SPHK1 (3G1) Monoclonal Antibody (bsm-54045R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Raji cells SPHK1 (3G1) Monoclonal Antibody (bsm-54045R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54045R
Product Name	SPHK1 (3G1) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human SPHK1 aa 50-150
Clonality	Monoclonal
Clone #	3G1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store for 1 year at -20C from date of shipment. Store for 1 year at -20C from date of shipment.
Target
Gene ID	8877
Swiss Prot	Q9NYA1
Synonyms	Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SPK
Background	Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400