CD163 (8G1) Monoclonal Antibody

$Human thymus tissue lysate probed with CD163 (8G1) Monoclonal Antibody (bsm-54015R)at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.\r\n\r\n$

Human thymus tissue lysate probed with CD163 (8G1) Monoclonal Antibody (bsm-54015R)at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.\r\n\r\n

$Paraformaldehyde-fixed and paraffin-embedded Human heart tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human heart tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded human liver tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin$

Paraformaldehyde-fixed and paraffin-embedded human liver tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin

$Paraformaldehyde-fixed and paraffin-embedded human placenta tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin$

Paraformaldehyde-fixed and paraffin-embedded human placenta tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin

$Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin$

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin

Flow cytometric analysis of thp-1 cells with CD163 (8G1) Monoclonal Antibody (bsm-54015R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: THP-1 (Negative control), 2: Human \r\nliver\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 121 kDa\r\nObserved MW: 150 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: THP-1 (Negative control), 2: Human \r\nliver\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 121 kDa\r\nObserved MW: 150 kDa

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Mouse liver, 2: Rat spleen\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 121 kDa\r\nObserved MW: 150 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Mouse liver, 2: Rat spleen\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 121 kDa\r\nObserved MW: 150 kDa

$Tissue: Human liver\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-54015R\r\n$

Tissue: Human liver\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-54015R\r\n

$Tissue: Human placenta\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-54015R\r\n$

Tissue: Human placenta\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-54015R\r\n

$Tissue: Mouse liver\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-54015R\r\n$

Tissue: Mouse liver\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-54015R\r\n

$Tissue: Rat liver\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-54015R\r\n$

Tissue: Rat liver\r\nSection type: Formalin-fixed & Paraffin -\r\nembedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:500\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-54015R\r\n

$Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Mouse liver, 2: Rat spleen\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 121 kDa\r\nObserved MW: 150 kDa$

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:5000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: 1: Mouse liver, 2: Rat spleen\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 121 kDa\r\nObserved MW: 150 kDa

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Rat

Overview
Catalog #	bsm-54015R
Product Name	CD163 (8G1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human CD163 between 1 to 250 amino acids
Clonality	Monoclonal
Clone #	8G1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	Q86VB7
Synonyms	Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD163, Soluble CD163, sCD163, CD163, M130
Background	Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC