Cathepsin B (3C9) Monoclonal Antibody

$Lane 1: HepG2; Lane 2: Hela; Lane 3:A549 lysate probed with Cathepsin B (3C9) Monoclonal Antibody (bsm-52977R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HepG2; Lane 2: Hela; Lane 3:A549 lysate probed with Cathepsin B (3C9) Monoclonal Antibody (bsm-52977R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Cathepsin B (3C9) Monoclonal Antibody (bsm-52977R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Cathepsin B (3C9) Monoclonal Antibody (bsm-52977R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Cathepsin B (3C9) Monoclonal Antibody (bsm-52977R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Cathepsin B (3C9) Monoclonal Antibody (bsm-52977R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Cathepsin B (3C9) Monoclonal Antibody (bsm-52977R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Cathepsin B (3C9) Monoclonal Antibody (bsm-52977R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52977R
Product Name	Cathepsin B (3C9) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Full length protein
Clonality	Monoclonal
Clone #	3C9
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1508
Swiss Prot	P07858
Synonyms	Cathepsin B, APP secretase, Cathepsin B1, Cathepsin B light chain, Cathepsin B heavy chain, APPS, CTSB
Background	Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed:12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed:12220505). Has also been implicated in tumor invasion and metastasis (PubMed:3972105).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200