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CD73 Recombinant Antibody

$A431 cells lysates probed with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

A431 cells lysates probed with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

$Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin$

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin

$Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin$

Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin

$Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin$

Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin

$Paraformaldehyde-fixed and paraffin-embedded Zebrafish tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin$

Paraformaldehyde-fixed and paraffin-embedded Zebrafish tissue incubated with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin

$IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

$IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

$IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:100 in NIH-3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

$Paraformaldehyde-fixed, paraffin embedded Human kidney tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD73 (2C9) Monoclonal Antibody, Unconjugated (bsm-52916R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD73 (2C9) Monoclonal Antibody, Unconjugated (bsm-52916R) at 1:200 overnight at 4°C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD73 (2C9) Monoclonal Antibody, Unconjugated (bsm-52916R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD73 (2C9) Monoclonal Antibody, Unconjugated (bsm-52916R) at 1:200 overnight at 4°C, DAB staining.

$25 ug total protein per lane of various lysates (see on figure) probed with CD73 monoclonal antibody, unconjugated (bsm-52916R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with CD73 monoclonal antibody, unconjugated (bsm-52916R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD73 Monoclonal Antibody, Unconjugated(bsm-52916R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$A431 cells lysates probed with CD73 (2C9) Monoclonal Antibody (bsm-52916R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Applications

WB
FCM
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Zebrafish

Overview
Catalog #	bsm-52916r
Product Name	CD73 Recombinant Antibody
Applications	WB, FCM, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human CD73 between 1 to 100 amino acids.
Clonality	Monoclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4907
Swiss Prot	P21589
Synonyms	5'-nucleotidase, Ecto-5'-nucleotidase, 5'-NT, CD73, NT5E
Background	Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200