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Lipocalin-2 (5C9) Monoclonal Antibody

Applications

  • WB
  • IHC-P
  • IF(ICC)
  • IHC

Reactivity

  • Human
Overview
Catalog # bsm-52914R
Product Name Lipocalin-2 (5C9) Monoclonal Antibody
Applications WB, IHC-P, IF(ICC), IHC
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source N terminal human Lipocalin-2
Clonality Monoclonal
Clone # 5C9
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 3934
Swiss Prot P80188
Synonyms Neutrophil gelatinase-associated lipocalin, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Lipocalin-2, NGAL, LCN2
Background Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC