Mannose Receptor(CD206) (1C4) Monoclonal Antibody

$Lane 1: Human lung lysates; Lane 2: HepG2 lysates; Line 3: 293T lysates; Probed with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Human lung lysates; Lane 2: HepG2 lysates; Line 3: 293T lysates; Probed with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Mannose Receptor(CD206) (1C4) Monoclonal Antibody (bsm-52791R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human 293T cell lysates; Lane 4: Human MCF-7 cell lysates ; Lane 5: Human Molt-4 cell lysates probed with Mannose Receptor(CD206) (1C4) Monoclonal Antibody, Unconjugated (bsm-52791R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$The Ramos(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-MRC1 antibody (bsm-52791R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The Ramos(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-MRC1 antibody (bsm-52791R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-52791r-100ul
Product Name	Mannose Receptor(CD206) (1C4) Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Full length protein
Immunogen Range	1400-1456/1456
Clonality	Monoclonal
Clone #	1C4
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4360
Swiss Prot	P22897
Synonyms	Macrophage mannose receptor 1, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor, Macrophage mannose receptor 1-like protein 1, CD_antigen: CD206, MMR, hMR, MRC1
Background	Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200