Tyrosine Hydroxylase (3T1) Monoclonal Antibody

Flow cytometric analysis of sh-sy5y cells with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: PC-12 Cells; Lane 2: Mouse brain; Probed with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: PC-12 Cells; Lane 2: Mouse brain; Probed with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:300 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:300 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:300 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:300 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Tyrosine Hydroxylase (3T1) Monoclonal Antibody (bsm-52689R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52689R
Product Name	Tyrosine Hydroxylase (3T1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human Tyrosine Hydroxylase between 500 to the C-terminus
Clonality	Monoclonal
Clone #	3T1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Swiss Prot	P07101
Synonyms	Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH
Background	Neuronal Marker Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC