MEK2 (2C1) Monoclonal Antibody

Flow cytometric analysis of HeLa cells with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: 293T Cells; Probed with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: 293T Cells; Probed with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:300 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:300 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with MEK2 (2C1) Monoclonal Antibody (bsm-52684R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52684R
Product Name	MEK2 (2C1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human MEK2 aa 1-100
Clonality	Monoclonal
Clone #	2C1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5605
Swiss Prot	P36507
Synonyms	Dual specificity mitogen-activated protein kinase kinase 2, ERK activator kinase 2, MAPK/ERK kinase 2, MAP kinase kinase 2, MAPKK 2, MEK 2, MAP2K2
Background	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC