Prion Protein (PrP) Recombinant Antibody

Flow cytometric analysis of SH-Sy5Y cells with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: Rat brain; Lane 2: Mouse brain; Probed with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C$

Lane 1: Rat brain; Lane 2: Mouse brain; Probed with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C

$IF(ICC) staining with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52505r-100ul
Product Name	Prion Protein (PrP) Recombinant Antibody
Applications	WB, FCM, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminal human Prion protein
Clonality	Monoclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5621
Swiss Prot	P04156
Synonyms	Major prion protein, ASCR, PrP27-30, PrP33-35C, CD_antigen: CD230, PrP, PRNP
Background	The function of PrP is still under debate. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis (By similarity). Isoform 2 may act as a growth suppressor by arresting the cell cycle at the G0/G1 phase. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200
IHC