PGK1 (8C12) Monoclonal Antibody

Flow cytometric analysis of HepG2 cells with PGK1 (8C12) Monoclonal Antibody (bsm-52475R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: Hela cells; Lane 2: HepG2 cells; Lane 3: MCF-7 cells; Probed with PGK1 (8C12) Monoclonal Antibody (bsm-52475R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela cells; Lane 2: HepG2 cells; Lane 3: MCF-7 cells; Probed with PGK1 (8C12) Monoclonal Antibody (bsm-52475R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with PGK1 (8C12) Monoclonal Antibody (bsm-52475R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PGK1 (8C12) Monoclonal Antibody (bsm-52475R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
FCM
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52475R
Product Name	PGK1 (8C12) Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human PGK1 between 1-100 amino acids
Clonality	Monoclonal
Clone #	8C12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5230
Swiss Prot	P00558
Synonyms	PhosphoglyceRate kinase 1, Cell migRation-inducing gene 10 protein, Primer recognition protein 2, PRP 2
Background	In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200