CDX2 Recombinant Antibody

AGS cell lysates probed with CDX2 (3G7) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.

Flow cytometric analysis of HeLa lysate probed with CDX2 (3G7) Monoclonal Antibody (bsm-52348R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$IF(ICC) staining with CDX2 (3G7) Monoclonal Antibody (bsm-52348R) at 1:100 in AGS cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with CDX2 (3G7) Monoclonal Antibody (bsm-52348R) at 1:100 in AGS cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with CDX2 (3G7) Monoclonal Antibody (bsm-52348R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with CDX2 (3G7) Monoclonal Antibody (bsm-52348R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat large intestine tissue incubated with CDX2 (3G7) Monoclonal Antibody (bsm-52348R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat large intestine tissue incubated with CDX2 (3G7) Monoclonal Antibody (bsm-52348R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Rat
Rabbit

Overview
Catalog #	bsm-52348r-100ul
Product Name	CDX2 Recombinant Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Rat, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human CDX2 between 1 to 150 amino acids
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1045
Swiss Prot	Q99626
Synonyms	Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2, CDX2
Background	Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC