ERG Recombinant Antibody

$Jurkat cell lysates probed with ERG (3F9) Monoclonal Antibody, unconjugated (bsm-52324R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n$

Jurkat cell lysates probed with ERG (3F9) Monoclonal Antibody, unconjugated (bsm-52324R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n

$Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with ERG (3F9) Monoclonal Antibody (bsm-52324R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with ERG (3F9) Monoclonal Antibody (bsm-52324R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with ERG (3F9) Monoclonal Antibody (bsm-52324R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with ERG (3F9) Monoclonal Antibody (bsm-52324R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Tissue: Mouse cerebrum\r\nSection type: Formalin-fixed & Paraffin-embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52324R$

Tissue: Mouse cerebrum\r\nSection type: Formalin-fixed & Paraffin-embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52324R

$Tissue: Rat kidney\r\nSection type: Formalin-fixed & Paraffin-embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52324R$

Tissue: Rat kidney\r\nSection type: Formalin-fixed & Paraffin-embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52324R

$Tissue: human kidney\r\nSection type: Formalin-fixed & Paraffin-embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52324R$

Tissue: human kidney\r\nSection type: Formalin-fixed & Paraffin-embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52324R

$Tissue: Human prostatic cancer\r\nSection type: Formalin-fixed & Paraffin-embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52324R$

Tissue: Human prostatic cancer\r\nSection type: Formalin-fixed & Paraffin-embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52324R

$25 ug total protein per lane of various lysates (see on figure) probed with ERG monoclonal antibody, unconjugated (bsm-52324R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ERG monoclonal antibody, unconjugated (bsm-52324R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52324r-100ul
Product Name	ERG Recombinant Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within Human ERG between 450 to C-terminus
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2078
Swiss Prot	P11308
Synonyms	Transcriptional regulator ERG, Transforming protein ERG, ERG
Background	Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC