STAT5a (6E5) Monoclonal Antibody

Lane 1: Hela; Lane 2: K562 cell lysates; probed with STAT5a (6E5) Monoclonal Antibody (bsm-52237R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

$Paraformaldehyde-fixed, paraffin embedded human tonsil section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with STAT5a (6E5) Monoclonal Antibody (bsm-52237R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human tonsil section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with STAT5a (6E5) Monoclonal Antibody (bsm-52237R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

HeLa cells were stained with STAT5a (6E5) Monoclonal Antibody (bsm-52237R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52237r-100ul
Product Name	STAT5a (6E5) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human STAT5a protein, around C-terminal 100aa.
Clonality	Monoclonal
Clone #	600000
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6776
Swiss Prot	P42229
Synonyms	MGF; STAT5; Signal transducer and activator of transcription 5A; STAT5A
Background	Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200