Phospholipase C gamma 1 (85F11) Monoclonal Antibody

SHG-44 cells were stained with Phospholipase C gamma 1 (85F11) Monoclonal Antibody (bsm-52174R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse NIH\/3T3 cell lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Testis tissue lysates; Lane 6: Human A431 cell lysates; Lane 7: Human A549 cell lysates; Lane 8: Human MCF-7 cell lysates; Lane 9: Human HeLa cell lysates probed with PLCG1 Monoclonal Antibody, Unconjugated (bsm-52174R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse NIH\/3T3 cell lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Testis tissue lysates; Lane 6: Human A431 cell lysates; Lane 7: Human A549 cell lysates; Lane 8: Human MCF-7 cell lysates; Lane 9: Human HeLa cell lysates probed with PLCG1 Monoclonal Antibody, Unconjugated (bsm-52174R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-52174R
Product Name	Phospholipase C gamma 1 (85F11) Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Phospholipase C gamma 1 protein, around C-terminal 150aa.
Clonality	Monoclonal
Clone #	85F11
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	5335
Swiss Prot	P19174
Synonyms	PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-148; Phosphoinositide phospholipase C-gamma-1; Phospholipase C-II; Phospholipase C-gamma-1; PLC-gamma-1; PLCG1
Background	Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Application Dilution
WB	1:500-2000