HDAC1 Recombinant Antibody

Lane 1: HeLa Cell lysates; Lane 2: Jurkat Cell lysates; Lane 3: K562 cell lysates; probed with HDAC1 (3C3) Monoclonal Antibody (bsm-52080R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

$Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with HDAC1 (3C3) Monoclonal Antibody (bsm-52080R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with HDAC1 (3C3) Monoclonal Antibody (bsm-52080R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human MOLT-4 cell lysates; Lane 4: Human U251 cell lysates probed with HDAC1 Monoclonal Antibody, Unconjugated bsm-52080R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human MOLT-4 cell lysates; Lane 4: Human U251 cell lysates probed with HDAC1 Monoclonal Antibody, Unconjugated bsm-52080R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52080r-100ul
Product Name	HDAC1 Recombinant Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human HDAC1 protein, around N-terminal 100aa.
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3065
Swiss Prot	Q13547
Synonyms	HD1; RPD3; GON-10; RPD3L1; Histone deacetylase 1; HDAC1
Background	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400