CRISPR-Cas9 (1E8) Monoclonal Antibody

Lane 1: 293T cells transfected with CRISPR9-Cas9; Lane 2: untransfected 293T cell lysate probed with CRISPR-Cas9 (1E8) Monoclonal Antibody, Unconjugated (bsm-52042M) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

Applications

Reactivity

Human
Others

Overview
Catalog #	bsm-52042m-100ul
Product Name	CRISPR-Cas9 (1E8) Monoclonal Antibody
Applications	WB
Reactivity	Human, Others
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (100-200aa)
Clonality	Monoclonal
Clone #	1E8
Isotype	IgM
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	901176
Swiss Prot	Q99ZW2
Synonyms	CRISPR; Cas9; CRISPR-associated endonuclease Cas9/Csn1; SpyCas9; SpCas9; csn1
Background	CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
Application Dilution
WB	1:300-5000