Get 20% off all conjugated antibody purchases with code "CON-ANTIBODY2025"! Offer valid on direct orders only. Available for US customers and internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Collagen 17 Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Collagen XVII (1C11) Monoclonal Antibody (bsm-52041R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Collagen XVII (1C11) Monoclonal Antibody (bsm-52041R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52041r
Product Name Collagen 17 Recombinant Antibody
Applications WB, IHC-P, IHC-F, IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human Collagen XVII protein, around 1300-1450aa.
Immunogen Range 1300-1450/1497
Clonality Monoclonal
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1308
Swiss Prot Q9UMD9
Synonyms Collagen alpha-1(XVII) chain; COL17A1
Background May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF

Search our catalog of hundreds of thousands of products