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ADAM17 Recombinant Antibody

SK-OV-3 cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS, blocked with 10% normal goat serum in PBS, and stained with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:200 in blocking solution. Slides were incubated overnight at 4\u00b0C followed by Cy5 conjugated secondary antibody incubation and DAPI staining of the nucleus.
SK-OV-3 cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS, blocked with 10% normal goat serum in PBS, and stained with ADAM17 (9E7) Monoclonal Antibody (bsm-52009R) at 1:200 in blocking solution. Slides were incubated overnight at 4°C followed by Cy5 conjugated secondary antibody incubation and DAPI staining of the nucleus.

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52009r
Product Name ADAM17 Recombinant Antibody
Applications WB, FCM, IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant human ADAM17 protein, around 700-824aa.
Immunogen Range 700-824/824
Clonality Monoclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6868
Swiss Prot P78536
Synonyms Disintegrin and metalloproteinase domain-containing protein 17; ADAM metallopeptidase domain 17; CSVP; TACE; NISBD; CD156B; NISBD1; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; Tumor Necrosis Factor Alpha Converting Enzyme.
Background Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(ICC) 1:50-200

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