Lamin A/C Monoclonal Antibody

$Lane 1: Hela cell lysates; Lane 2: C6 cell lysates; Lane 3: L929 cell lysates; Lane 4: NIH\/3T3 cell lysates probed with Lamin A\/C Monoclonal Antibody, Unconjugated (bsm-51683M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Hela cell lysates; Lane 2: C6 cell lysates; Lane 3: L929 cell lysates; Lane 4: NIH\/3T3 cell lysates probed with Lamin A\/C Monoclonal Antibody, Unconjugated (bsm-51683M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51683M
Product Name	Lamin A/C Monoclonal Antibody
Applications	WB
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human Lamin A/C.
Clonality	Monoclonal
Clone #	C3S4
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4000
Swiss Prot	P02545
Subcellular location	Nucleus
Synonyms	Lamin A + Lamin C; Lamin A + C; LMN1; 70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; HGPS; IDC; LAMIN A; lamin A/C; LAMIN C; LDP1; LFP; LGMD1B; LMN 1; LMN A; LMN C; LMNA; LMNC; NY REN 32 antigen; PRO1; LMNA_HUMAN; Prelamin-A/C; Renal carcinoma antigen NY-REN-32.
Background	Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Play an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Prelamin-A/C can accelerate smooth muscle cell senescence. It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence.
Application Dilution
WB	=1:500-2000Ôºå