GLS2 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLS2 Monoclonal Antibody, Unconjugated (bsm-51645M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLS2 Monoclonal Antibody, Unconjugated (bsm-51645M) at 1:25 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P

Predicted Reactivity

Human
Mouse

Overview
Catalog #	bsm-51645M
Product Name	GLS2 Monoclonal Antibody
Applications	WB, IHC-P
Predicted Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human GLS2.
Clonality	Monoclonal
Clone #	Y09A1
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	27165
Swiss Prot	Q9UI32
Subcellular location	Cytoplasm
Synonyms	breast cell glutaminase; GLSL_HUMAN; glutaminase 2 (liver mitochondrial); glutaminase 2; glutaminase GA; glutaminase I; Glutaminase liver isoform; hLGA; L glutaminase; L glutamine amidohydrolase; L-glutaminase; L-glutamine amidohydrolase; LGA; mitochondrial; phosphate activated glutaminase; phosphate-dependent glutaminase.
Background	Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Application Dilution
WB	=1:500-2000ÔºåIHC-P=1:25ÔºåIHC-F=1:25Ôºå
IHC-P	1:200-400