Erk2 Monoclonal Antibody

$Lane 1: MCF-7 cell lysates; Lane 2: HepG2 cell lysates; Lane 3: NCI-H292 cell lysates; Lane 4: Hela cell lysates; Lane 5: NIH\/3T3 cell lysates; Lane 6: Neuro-2a cell lysates; Lane 7: C6 cell lysates probed with Erk2 Monoclonal Antibody, Unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: MCF-7 cell lysates; Lane 2: HepG2 cell lysates; Lane 3: NCI-H292 cell lysates; Lane 4: Hela cell lysates; Lane 5: NIH\/3T3 cell lysates; Lane 6: Neuro-2a cell lysates; Lane 7: C6 cell lysates probed with Erk2 Monoclonal Antibody, Unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Hela cell lysates; Lane 2: Hela-MAPK1 KO cell lysates probed with Erk2 Monoclonal Antibody, Unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Hela cell lysates; Lane 2: Hela-MAPK1 KO cell lysates probed with Erk2 Monoclonal Antibody, Unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Xenopus laevis

Predicted Reactivity

Human
Mouse
Rat
Cow

Overview
Catalog #	bsm-51555M
Product Name	Erk2 Monoclonal Antibody
Applications	WB
Reactivity	Xenopus laevis
Predicted Reactivity	Human, Mouse, Rat, Cow
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human Erk2
Immunogen Range	101-200/360
Clonality	Monoclonal
Clone #	D2F12
Isotype	IgG1 k
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5594
Swiss Prot	P28482
Subcellular location	Extracellular, Cytoplasm, Nucleus, Cell membrane
Synonyms	ERK 2; ERK-2; ERT1; Extracellular Signal Regulated Kinase 2; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; Mapk1; Mitogen-activated protein kinase 1; MK01_HUMAN; P38; P40; P41; p42-MAPK; P42MAPK; PRKM1; PRKM2; protein kinase, mitogen-activated, 1; protein kinase, mitogen-activated, 2; protein tyrosine kinase ERK2.
Application Dilution
WB	=1:500-2000Ôºå