VCP (4C4) Monoclonal Antibody

$Lane 1: rat liver lysates; Lane 2: NIH\/3T3 Cell lysates; Lane 3: MCF-7 Cell lysates; Lane 4: mouse liver lysates; Lane 5: C6 Cell lysates;probed with VCP (4C4) Monoclonal Antibody, unconjugated (bsm-51420M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: rat liver lysates; Lane 2: NIH\/3T3 Cell lysates; Lane 3: MCF-7 Cell lysates; Lane 4: mouse liver lysates; Lane 5: C6 Cell lysates;probed with VCP (4C4) Monoclonal Antibody, unconjugated (bsm-51420M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Fluorescent image of U251 cells stained with VCP (4C4) Antibody (bsm-51420M) at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor\u00ae 555 conjugated with Phalloidin (red).$

Fluorescent image of U251 cells stained with VCP (4C4) Antibody (bsm-51420M) at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor\u00ae 555 conjugated with Phalloidin (red).

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51420M
Product Name	VCP (4C4) Monoclonal Antibody
Applications	WB, FCM, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This VCP antibody is generated from a mouse immunized with a recombinant protein from human VCP.
Clonality	Monoclonal
Clone #	4C4
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7415
Swiss Prot	P55072
Synonyms	p97; TERA; ALS14; CMT2Y; IBMPFD; HEL-220; IBMPFD1; HEL-S-70; Transitional endoplasmic reticulum ATPase; TER ATPase; 15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein; VCP
Background	Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF