NFKBIA (1C1) Monoclonal Antibody

$Lane 1: Hela Cell lysates; Lane 2: NIH\/3T3 Cell lysates; probed with NFKBIA (1121CT8.6.1) Monoclonal Antibody, unconjugated (bsm-51393M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela Cell lysates; Lane 2: NIH\/3T3 Cell lysates; probed with NFKBIA (1121CT8.6.1) Monoclonal Antibody, unconjugated (bsm-51393M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51393M
Product Name	NFKBIA (1C1) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged NFKBIA protein was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	1C1
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4792
Swiss Prot	P25963
Synonyms	IKBA; MAD-3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; MAD3
Background	Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Application Dilution
WB	1:300-5000