EIF2AK3 (3C3) Monoclonal Antibody

$Lane 1: A549; Lane 2: PC-12; Lane 3: mouse brain; Lane 4: mouse liver cell lysate at 20 \u00b5g per lane, probed with bsm-51385M EIF2AK3 (3C3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: A549; Lane 2: PC-12; Lane 3: mouse brain; Lane 4: mouse liver cell lysate at 20 \u00b5g per lane, probed with bsm-51385M EIF2AK3 (3C3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Lane 1: SY5Y cell lysates probed with EIF2AK3 (3C3) Monoclonal Antibody, Unconjugated (bsm-51385M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Mouse Cerebrum lysates probed with EIF2AK3 (3C3) Monoclonal Antibody, Unconjugated (bsm-51385M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse NIH\/3T3 cell lysates probed with EIF2AK3 Monoclonal Antibody, Unconjugated (bs-51385M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse NIH\/3T3 cell lysates probed with EIF2AK3 Monoclonal Antibody, Unconjugated (bs-51385M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51385M
Product Name	EIF2AK3 (3C3) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This EIF2AK3 antibody is generated from a mouse immunized with a recombinant protein conjugated synthetic peptide between 530-850 amino acids from human EIF2AK3.
Clonality	Monoclonal
Clone #	3C3
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9451
Swiss Prot	Q9NZJ5
Synonyms	PEK; WRS; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; EIF2AK3
Background	Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400