CDH1 (9C8) Monoclonal Antibody

$Lane 1: A431 Cell lysates; Lane 2: T47D Cell lysates; probed with CDH1 (9C8) Monoclonal Antibody, unconjugated (bsm-51351M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: A431 Cell lysates; Lane 2: T47D Cell lysates; probed with CDH1 (9C8) Monoclonal Antibody, unconjugated (bsm-51351M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded human colon tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDH1 (9C8) Monoclonal Antibody (bsm-51351M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human colon tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDH1 (9C8) Monoclonal Antibody (bsm-51351M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

$Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized MCF-7 cells; antibody incubation with CDH1 (9C8) Monoclonal Antibody, Unconjugated (bsm-51351M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary DAPI and for nuclei staining.$

Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized MCF-7 cells; antibody incubation with CDH1 (9C8) Monoclonal Antibody, Unconjugated (bsm-51351M) at 1:25 overnight at 4\u00b0C, followed by a conjugated secondary DAPI and for nuclei staining.

Applications

WB
FCM
IHC-P
IF(IHC-P)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-51351M
Product Name	CDH1 (9C8) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This CADH1 antibody is generated from a mouse immunized with a recombinat protein between 1-392 amino acids from human CADH1.
Immunogen Range	1-392/882
Clonality	Monoclonal
Clone #	9C8
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	999
Swiss Prot	P12830
Synonyms	UVO; CDHE; ECAD; LCAM; Arc-1; CD324; Cadherin-1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CDH1
Background	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(IHC-P)	1:50-200
IF(ICC)	1:50-200