MITF (M2A1) Monoclonal Antibody

$Lane 1: A431; Lane 2: PC-12; Lane 3: NIH\/3T3 cell lysate (20\u00b5g per lane) probed with bsm-51339M MITF (M2A1) Monoclonal Antibody at 1:2000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: A431; Lane 2: PC-12; Lane 3: NIH\/3T3 cell lysate (20\u00b5g per lane) probed with bsm-51339M MITF (M2A1) Monoclonal Antibody at 1:2000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

Lane 1: A431 cell lysates probed with MITF (M2A1) Monoclona Antibody, Unconjugated (bsm-51339M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51339M
Product Name	MITF (M2A1) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This MITF antibody is generated from a mouse immunized with a recombinant protein of human MITF.
Clonality	Monoclonal
Clone #	M2A1
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4286
Swiss Prot	O75030
Synonyms	MI; WS2; CMM8; WS2A; bHLHe32; Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; MITF
Background	Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Application Dilution
WB	1:300-5000