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RFA2 (5C3) Monoclonal Antibody

Applications

  • WB
  • IHC-P

Reactivity

  • Human
Overview
Catalog # bsm-51336M
Product Name RFA2 (5C3) Monoclonal Antibody
Applications WB, IHC-P
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source This RFA2 antibody is generated from a mouse immunized with a recombinant protein of human RFA2.
Clonality Monoclonal
Clone # 5C3
Isotype IgG1
Concentration 0.5ug/ul
Purification Purified by Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 6118
Swiss Prot P15927
Synonyms REPA2; RPA32; RP-A p32; RP-A p34; Replication protein A 32 kDa subunit; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RPA2; RPA34
Background As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400